Dot-it-Spot-it Total Protein Assay

Dot-it-spot-it shows response in the range 0.1 – 5 ng BSA per dot. Using 0.5 µL sample + 0.5 µL dilution buffer per dot, the test can quantify in the range 0.2-10 µg BSA/mL.

Compare to other total protein tests:  Pierce Coomassie (Bradford) Protein Assay with Micro Microplate Protocol shows response in the range 150 – 3750 ng BSA per well. Using 150 µL sample + 150 µL reagent per well, the test can quantify in the range 1-25 µg BSA/mL.

Dot-it-Spot-it is an ultra-sensitive total protein assay!
Dot-it-Spot-it is an easy-to-use kit!


Dot-it-Spot it is available as a kit containing detection sheets, and dilution and detection solutions. Just apply 1 µL sample mixture on the detection sheet, dry and detect.   Read more…



The results are quantified by the use of an image scanner, which measures delta blackness per pixel in the spot.   Read more…


March 2019:  We introduce a new kit for 18 dots per detection sheet.