Dot-it-Spot-it Total Protein Assay

Dot-it-spot-it shows response in the range 0.1 – 5 ng BSA per dot. Using 0.5 µL sample + 0.5 µL dilution buffer per dot, the test can quantify in the range 0.2-10 µg BSA/mL.

Compare to other total protein tests:  Pierce Coomassie (Bradford) Protein Assay with Micro Microplate Protocol shows response in the range 150 – 3750 ng BSA per well. Using 150 µL sample + 150 µL reagent per well, the test can quantify in the range 1-25 µg BSA/mL.

Dot-it-Spot-it is an ultra-sensitive total protein assay!
Dot-it-Spot-it is an easy-to-use kit!

Method

Dot-it-Spot it is available as a kit containing detection sheets, and dilution and detection solutions. Just apply 1 µL sample mixture on the detection sheet, dry and detect.   Read more…

Results

130930-HSA-standard-web

The results are quantified by the use of an image scanner, which measures delta blackness per pixel in the spot.   Read more…

Updates

March 2019:  We introduce a new kit for 18 dots per detection sheet.