Dot-it-Spot-it Total Protein Assay

2015-08-20 This sensitive and rapid total protein assay works well with both detergents and reducing substances (like DTT) !

One big area for interest is the quantification of proteins in cerebrospinal fluid (CSF), after depletion of several abundant proteins. The main amount of protein is required for the MS analysis, and only minute protein amounts (nanograms!)  can be used for estimation of total protein.

”As a researcher, I am happy to offer you a new product that I have longed-for in decades !  This tool helps me to check up every step when I handle low amounts of proteins.”   / Maria Lönnberg at Uppsala University, Sweden

 

“Dot-it-Spot-it is an ultra-sensitive protein assay !”

Method

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Dot-it-Spot-it is available as a kit containing detection sheets, a detection solution with carbon black particles, and a washing solution. Just apply and dry 1 uL sample on the detection sheet, dip it into the black detection solution, wash, dry and scan. This takes less then 10 minutes.

Results

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 The results are quantified by the use of an image scanner, which measures the blackness intensity in the spots. On the x-axis is shown albumin concentration from 0.3 to 20 ug/mL, and on the y-axis is the intensity signal measured as delta blackness per pixel.

 

 

 

Applications

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Dot-it-Spot-it rapidly shows the protein concentration in your samples so you can select the optimal application amount for electrophoresis or mass spectrometry analysis.

Dot-it-Spot-it requires only around 1-2 nanogram of protein for estimation of concentration. This  saves your valuable biological samples to the subsequent analytical steps.

 

 

Dot-it-Spot-it is rapid!          Dot-it-Spot-it is easy-to-use!