Method

Dot-it-Spot-it®  Total Protein Assay

Downloads

Instructions for Assay – March 2024
Template for scanner Calculation for 20 dots – June 2023

The Dot-it-Spot-it kit contains four reagents

  • Detection Sheet
  • Dilution Buffer
  • Detection Solution
  • Washing Solution

SampleMix

Nano method: 50% sample, 0.2 µg BSA/mL
Micro method: 5% sample, 2 µg BSA /mL

Step by step

Method_1_01-01b

  • Detection Sheet
  • Mix sample with Dilution Buffer (= Application mix)
  • Apply 1 µL Application mix
  • Dry

Method_1_01-02b

  • 1.5 mL Detection Solution
  • 5 min migration
  • 1 mL Washing Solution
  • 5 min migration

Method_1_01-03b

  • Dry and mount
  • Scan with an image scanner
  • Quantify the blackness intensity