Method

Dot-it-Spot-it® Protein Assay

Step by step

Method_1_01-01b

  • Detection sheet
  • Mix sample with Dilution buffer (= Application mix)
  • Apply 1 µL Application mix
  • Dry

Method_1_01-02b

  • 1 mL Detection solution
  • 5 min migration
  • 1 mL Washing solution
  • 5 min migration

Method_1_01-03b

  • Dry and mount
  • Scan with an image scanner
  • Quantify the blackness intensity

Detection tools

Method_3_01

Method_3_02

Method_3_03

March 2015. Download the file “Detection recommendations for Dot-it-Spot-it protein assay, 01.03.2015” showing the way to use eye, smartphone and scanner for detection.

We recommend use of the affordable scanner Epson Perfection V600 Photo, together with the software Epson Scan.

We also tested iPhone 5s as detection tool with good results.