Method
Dot-it-Spot-it® Total Protein Assay
The Dot-it-Spot-it kit contains four reagents
- Detection Sheet
- Dilution Buffer
- Detection Solution
- Washing Solution
Step by step

- Detection Sheet
- Mix sample with Dilution Buffer (= Application mix)
- Apply 1 µL Application mix
- Dry

- 1 mL Detection Solution
- 5 min migration
- 1 mL Washing Solution
- 5 min migration

- Dry and mount
- Scan with an image scanner
- Quantify the blackness intensity