Applications
Dot-it-Spot-it® Total Protein Assay
Dot-it-Spot-it is useful for CHROMATOGRAPHY detection
Low amounts of protein cannot be detected with A280!
Dot-it-Spot-it test for control of HSA depletion column
Depleted Flow-through fractions:
97 µg Fetuin and < 1.5 µg HSA.
From these valuable 1 mL fractions you need only a few µL for testing.
Eluted fractions:
162 µg HSA and 1.7 µg Fetuin
Dot-it-Spot-it is useful for PRE-TEST control
Depletion of cerebrospinal fluid (CSF) as a pre-step to MS analysis
CSF – check for high protein levels, it may be due to traces of blood proteins.
Follow your proteins in every pre-step.
With Dot-it-Spot-it assay you don’t waste material.
With Dot-it-Spot-it assay you can easily run several hundreds of samples in short time.