Reference

Dot-it-Spot-it® Protein Assay

Studies that have been using Dot-it-Spot-it Protein Assay

2014  Intracellular concentration of the tyrosine kinase inhibitor imatinib in gastrointestinal stromal tumor cells.
Anticancer Drugs. 2014 Apr;25(4):415-22.
Berglund E, Ubhayasekera SJ, Karlsson F, Akcakaya P, Aluthgedara W, Ahlen J, Fröbom R, Nilsson IL, Lui WO, Larsson C, Zedenius J, Bergquist J, Bränström R.

2015  High-abundant protein depletion strategies applied on dog cerebrospinal fluid and evaluated by high-resolution mass spectrometry.
Biochemistry and Biophysics Reports. 2015, 3, 68-75.
Sundberg, M., Bergquist, J., Ramström, M.

2015  A proteomic approach to monitor the dynamic response of the female oviductal epithelial cell surface to male gametes.
Journal of proteomics. 2015, 113, 1-14.
Artemenko, K., Horakova, J., Steinberger, B., Besenfelder, U., Brem, G., Bergquist, J., Mayrhofer, C.

2016  Analysis of the cerebrospinal fluid proteome in Alzheimers´s disease.
PLOS ONE. 11(3) e0150672.  DOI:10.1371/journal.pone.0150672 March 7, 2016
Khoonsari, P.E., Häggmark, A., Lönnberg, M., Mikus, M., Kilander, L., Lannfelt, L., Bergquist, J., Ingelsson, M., Nilsson, P., Kultima, K., Shevchenko, G (2016).

2016  Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins.
Analyst. 2016, 141, 981-988.
Duong-Thi, M-D., Bergström, M., Edwards, K., Eriksson, J., Ohlson, S., To Yiu Ying, J., Torres, J., Agmo Hernández, V.

2017   Combined lipidomic and proteomic analysis of isolated human islets exposed to palmitate reveals time-dependent changes in insulin secretion and lipid metabolism.
PLoS ONE. 12(4): e0176391. 2016, 141, 981-988.
Kirsten Roomp , Hjalti Kristinsson , Domitille Schvartz, Kumari Ubhayasekera, Ernest Sargsyan, Levon Manukyan, Azazul Chowdhury, Hannes Manell, Venkata Satagopam, Karlfried Groebe, Reinhard Schneider, Jonas Bergquist, Jean-Charles Sanchez, Peter Bergsten

2018  Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.
Nature Scientific Reports volume 8, Article number: 5400 (2018). DOI:10.1038/s41598-018-23582-1.
Klaesson A.1, Grannas K.1, Ebai T.2, Heldin J.1, Koos B.3, Leino M.1, Raykova D.1, Oelrich J.2, Arngården L.1, Söderberg O.1, Landegren U.2.
1 Department of Pharmaceutical Biosciences, Pharmaceutical Cell Biology, Uppsala University, Uppsala, Sweden.
2 Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
3 Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Ola Söderberg and Ulf Landegren jointly supervised this work.

2018   Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells.
Oncogene (2018), 37:2515–2531.
Laia Caja1,2, Kalliopi Tzavlaki1,2, Mahsa S. Dadras1,2, E-Jean Tan3, Gad Hatem1,2, Naga P. Maturi1,2,3, Anita Morén1,2, Lotta Wik4, Yukihide Watanabe2, Katia Savary2, Masood Kamali-Moghaddan4, Lene Uhrbom3, Carl-Henrik Heldin1,2, Aristidis Moustakas1,2.
1Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Box 582, Biomedical Center, Uppsala University, SE-75123 Uppsala, Sweden
2 Ludwig Cancer Research, Science for Life Laboratory, Box 595, Biomedical Center, Uppsala University, SE-75124 Uppsala, Sweden.
3 Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Rudbeck Laboratory, Uppsala University, SE- 75185 Uppsala, Sweden.
4 Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Box 815, Biomedical Center, Uppsala University, SE-75108 Uppsala, Sweden.

2019   Systematic analysis of the cerebrospinal fluid proteome of fibromyalgia patients.
Journal of Proteomics Volume 190, 6 January 2019, Pages 35-43.
Payam EmamiKhoonsaria, Sravani Musunrib,Stephanie Hermana,Camilla I.Svenssonc,Lars Tanumdd,TorstenGordhe,Kim Kultimaac.
a Department of Medical Sciences, Clinical Chemistry, Uppsala University, Uppsala, Sweden.
b Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, Uppsala, Sweden.
c Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
d Department of R&D in Mental Health, Akershus University Hospital, Lørenskog, Norway.
e Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.

2019   Profiling surface proteins on individual exosomes using a proximity barcoding assay.
NATURE COMMUNICATIONS | (2019) 10:3854.
Di Wu1,2,3, Junhong Yan1, Xia Shen4,5,6, Yu Sun7,11, Måns Thulin8,12, Yanling Cai9, Lotta Wik1, Qiujin Shen1, Johan Oelrich3, Xiaoyan Qian2, K. Louise Dubois10, K. Göran Ronquist10, Mats Nilsson2, Ulf Landegren1, Masood Kamali-Moghaddam1.
1 Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-75 185 Uppsala, Sweden.
2 Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, SE-17 165 Solna, Sweden.
3 Vesicode AB, Nobels väg 16, Solna SE-17 165, Sweden.
4 Center for Global Health Research, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK.
5 Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Nobels väg 12 A, Stockholm SE-17 177, Sweden.
6 BiostatisticsGroup, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou CN 510000, China.
7 Department of Molecular Evolution, Uppsala University, SE-751 24 Uppsala, Sweden.
8 Department of Statistics, Uppsala University, SE- 75 120 Uppsala, Sweden.
9 Institute of Translational Medicine, The Second Peopleʹs Hospital of Shenzhen, Shenzhen CN 518000, China.
10 Department of Medical Sciences, Clinical Chemistry, Uppsala University, SE-75 185 Uppsala, Sweden.
11 Present address: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China.
12 Present address: School of Mathematics and Maxwell Institute for Mathematical Sciences, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG, UK.

2020   Human proteins incorporated into tick-borne encephalitis virus revealed by in situ proximity ligation.
Biochemical and Biophysical Research Communications 525 (2020) 714e719.
Ryoyo Ikebuchiab, Alfred W. Isaaca, Kentaro Yoshiic, Ehsan Manouchehri Doulabia, Liza Löfa, Alireza Azimia, Lei Chena, Claudia Fredolinia, Radiosa Gallinia, Ulf Landegrena, Masood Kamali-Moghaddama.
a Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
b JSPS Overseas Research Fellow, Japan Society for the Promotion of Science, Japan.
c Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.