Dot-it-Spot-it Total Protein Assay
Dot-it-Spot-it Total Protein Assay needs less than nanogram protein for analysis!
Dot-it-spot-it shows response in the range 0.15 – 5 ng BSA per dot.
Using 0.5 µL sample + 0.5 µL Dilution buffer per dot, the test can quantify in the range 0.3-10 µg BSA/mL.
All you need is a photo scanner to make quantitative detection.
Dot-it-Spot-it is an ultra-sensitive total protein assay!
Dot-it-Spot-it is an easy-to-use kit!
Method
Dot-it-Spot it is available as a kit containing detection sheets, and dilution and detection solutions. Just apply 1 µL sample mixture on the detection sheet, dry and detect. Read more…
Results
The results are quantified by the use of an image scanner, which measures delta blackness per pixel in the spot. Read more…
Updates
January 2021: Updated Reference list.