Dot-it-Spot-it® Protein Assay

Human Albumin (HSA)

Results fot HSA 0.16-10 ug/ml = 0.2-10 ng.

Sample: HSA, Sigma A8763 diluted in 1% SDS, 20 mM TRIS pH 7,5, 0,15 M NaCl, 0.02 % NaN3.

A. Semiquantitative detection by eye.

B. Quantitative detection with scanner, Epson Perfection V600 Photo, using Epson Scan software.


microgram/mL or nanogram HSA



Studies that have been using Dot-it-Spot-it Protein Assay

1. Intracellular concentration of the tyrosine kinase inhibitor imatinib in gastrointestinal stromal tumor cells.  Anticancer Drugs. 2014 Apr;25(4):415-22.
Berglund E, Ubhayasekera SJ, Karlsson F, Akcakaya P, Aluthgedara W, Ahlen J, Fröbom R, Nilsson IL, Lui WO, Larsson C, Zedenius J, Bergquist J, Bränström R.

3. High-abundant protein depletion strategies applied on dog cerebrospinal fluid and evaluated by high-resolution mass spectrometry.  Biochemistry and Biophysics Reports. 2015, 3, 68-75.
Sundberg, M., Bergquist, J., Ramström, M.

5. Analysis of the cerebrospinal fluid proteome in Alzheimers´s disease.  PLOS ONE. 11(3) e0150672.  DOI:10.1371/journal.pone.0150672
Khoonsari, P.E., Häggmark, A., Lönnberg, M., Mikus, M., Kilander, L., Lannfelt, L., Bergquist, J., Ingelsson, M., Nilsson, P., Kultima, K., Shevchenko, G (2016).


2. A proteomic approach to monitor the dynamic response of the female oviductal epithelial cell surface to male gametes.  Journal of proteomics. 2015, 113, 1-14.
Artemenko, K., Horakova, J., Steinberger, B., Besenfelder, U., Brem, G., Bergquist, J., Mayrhofer, C.

4. Lipodisks integrated with weak affinity chromatography enable fragment screening of integral membrane proteins.  Analyst. 2016, 141, 981-988.
Duong-Thi, M-D., Bergström, M., Edwards, K., Eriksson, J., Ohlson, S., To Yiu Ying, J., Torres, J., Agmo Hernández, V.