Method
Dot-it-Spot-it® Total Protein Assay
Downloads
Instructions for Assay – March 2024
16 bit Scanning Template for Calculation – Nov 2024
8 bit Scanning Template for Calculation – Nov 2024
The Dot-it-Spot-it kit contains four reagents
- Detection Sheet
- Dilution Buffer
- Detection Solution
- Washing Solution
Nano method: 50% sample, 0.2 µg BSA/mL
Micro method: 5% sample, 2 µg BSA /mL
Step by step
- Detection Sheet
- Mix sample with Dilution Buffer (= Application mix)
- Apply 1 µL Application mix
- Dry
- 1.5 mL Detection Solution
- 5 min migration
- 1 mL Washing Solution
- 5 min migration
- Dry and mount
- Scan with an image scanner
- Quantify the blackness intensity